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Features of the blood biochemical composition and adsorption-transport function of erythrocytes in the grey seal (Halichoerus grypus Fabricius, 1791) in the early postnatal period of development. P. 123–131

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Section: Biology

UDC

599.745.3: 591.111.1

Authors

IA Erokhina1, NN Kavtsevich1
1 Murmansk Marine Biological Institute of the Russian Academy of Sciences (Murmansk, Russian Federation)
Corresponding author: Irina Erokhina (irina.erohina58@mail.ru)

Abstract

The results of the study of certain biochemical parameters of blood in grey seals (Halichoerus grypus Fabricius, 1791) from birth to weaning are presented. In the blood plasma, 20 indices describing the state of the metabolism of proteins, carbohydrates, lipids, minerals (total protein, urea, creatinine, glucose, lactic acid, total lipids, triglycerides, cholesterol, calcium, phosphorus, sodium, potassium, magnesium, iron, aspartate aminotransferase, alanine aminotransferase, γ-glutamyltransferase, creatine kinase, alkaline phosphatase, lactate dehydrogenase) are studied. It has been established that metabolic changes in the early period of postnatal development of grey seals occur in mammals in general, but the expression level of individual reactions may be considered a feature of pinnipeds. By the ratio of transaminase activity, the predominance of catabolism over anabolism was established in animals in the studied period of development. The most significant changes in the metabolism of grey seals were noted during weaning and the transition to self-feeding. The adsorption- transport function of erythrocytes was evaluated by washout on the erythrocyte membranes using 3% sodium chloride solution. It was established that all detected components of blood plasma are found in erythrocyte washings. The level of adsorption, expressed as a percentage of the content of a certain metabolite in the blood plasma, is not the same for different compounds. Enzymes, urea, creatinine, lipids are adsorbed most on the erythrocyte membranes (in some cases more than 100%), and glucose, lactic acid, cholesterol, iron, calcium are adsorbed in a smaller quantity (<50%). The data obtained indicate that the transfer of organic compounds on erythrocytes is more variable and demonstrative than shifts in the corresponding blood plasma parameters, and erythrocytes can participate in maintaining a range of concentrations of a number of substances in the blood plasma.

Keywords

metabolic adaptation of the grey seal, biochemical composition of the grey seal blood, adsorption-transport function of erythrocytes
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References

  1. Boily F, Beaudoin S, Measures LN (2006) Haematology and serum chemistry of harp (Phoca groenlandica) and hooded seals (Cystophora crystata) during the breeding season, in the Gulf of St. Lawrence, Canada. Journal of Wildlife Diseases 42(1): 115–132. https://doi.org/10.7589/0090-3558-42.1.115
  2. Bossart GD, Reidarson TH, Dierauf LA, Duffeld DA (2001) Clinical pathology. In: Dierauf LA, Gulland FVD (Eds) CRC Handbook of marine mammal medicine (2nd edn). CRC Press, 383–436. https://doi.org/10.1201/9781420041637. sec4
  3. Cohen CM, Solomon AK (1976) Ca binding to the human red cell membrane: characterisation of membrane preparations and binding sites. Journal of Membrane Biology 29: 345–372. https://doi.org/10.1007/BF01868970
  4. Danilova LA (2003) A Guide for Laboratory Study Methods. Peter, Saint Petersburg, 736 pp.
  5. Dmitrieva LA, Kirdey EG (1995) The nature and terms of sorption by erythrocytes of biologically active substances. Siberian Medical Journal (Irkutsk) 3(2): 23–25.
  6. Dover SD, McBain DVM, Little K (1993) Serum alkaline phosphatase as an indicator of nutritional status in cetaceans. In: Proceedings of the International Association of Aquatic Animal Medicine 24: 44.
  7. Ermolina SA, Sozinov VA (2005) Changes in the levels of serum iron and haemoglobin of standard mink youngsters in the age dynamics. In: Physiological bases of increased productivity of mammals introduced in zooculture. Proceedings of the International Symposium, Petrozavodsk (the Republic of Karelia, Russia), September 2005. Petrozavodsk, 64–66.
  8. Erokhina IA (2007) Biochemical parameters of blood plasma of the harp seal Pagophilus groenlandicus Erxleben, 1777 (Pinnipedia, Phocidae) of different age. Journal of Evolutionary Biochemistry and Physiology 43(3): 254–257.https://doi.org/10.1134/S0022093007030040
  9. Erokhina IA (2009) Characteristics of metabolism of the grey seal (Halichoerus grypus Fabricius, 1791) in the early postnatal period of development. Reports of the Academy of Sciences 424(3): 419–421.
  10. Fulbright RM, Axelrod D (1993) Dynamics of nonspecific adsorption of insulin to erythrocyte membranes. Journal of Fluorescence 3(1): 1–16.https://doi.org/10.1007/BF00865284
  11. Gareyev RA (1999) The second important function of erythrocytes. Physiological bases of a healthy lifestyle – Materials of the 4th Congress of Physiologists of Kazakhstan, Astana, 111–115.
  12. Gareyev RA (2011) Fundamental and applied aspects of the adsorption-transport function of erythrocytes. Health. Medical Ecology Science 2(45): 22–24.
  13. Gareyev RA, Murzamadiyeva AA, Sadykova HM, Achmetova BS, Fyzulina FR (1998) Technique of analysis of the output of glucose from blood into tissue. In: Biological Motility: Modern Methods for Studying. Pushchino, 43–44.
  14. Geraci JR, Smith TG (1975) Functional hematology of ringed seals (Phoca hispida) in the Canadian Arctic. Journal of the Fisheries Research Board of Canada 32: 2559–2564. https://doi.org/10.1139/f75-302
  15. Kikuchi Y, Koyama T (1984) Red blood cell deformability and protein absorbtion on red blood cell surface. American Journal of Physiology 247: 739–747.
  16. Kumar D, Rizvi SI (2013) Erythrocyte membrane bound and plasma sialic acid during aging. Biologia 68(4): 762–765. https://doi.org/10.2478/s11756-013-0207-1
  17. Meyer DJ, Harvey JW (1998) In: Veterinary Laboratory Medicine: Interpretation and Diagnosis. Philadelphia, 157–186.
  18. Minzyuk TV (2017) Morphometric parameters of erythrocytes in grey and harp seals. In: Research on the ecosystems of the Arctic seas: Materials of Conference of Young Scientists of MMBI KSC RAS, dedicated to the Year of Ecology in Russia, Murmansk (Russia), May 2017. Publishing house of the MMBI KSC RAS, Murmansk, 55–60.
  19. Oshakbayev KP, Khan OP, Kozhabekova BN, Seitbai AN, Dukenbaeva BA, Ischanova GR (2007) Relationship between endogenous intoxication and anaemia. Preventive Medicine 1: 21–25.
  20. Rosliy IM, Vodolazhskaya MG (2010) Rules for Reading Biochemical Analysis: A Physician’s Guide. Medical Informational Agency, Moscow, 96 pp.
  21. Troshkina ON, Tsirkin VI, Dvoryanskiy SA (2007) Erythrocyte: the structure and function of its membrane. Vyatka Medical Bulletin 2–3: 32–40.
  22. Zaitsev GN (1991) Mathematical Analysis of Biological Data. Moscow, 184 pp.
  23. Zbarsky BI, Demin NN (1949) The Role of Erythrocytes in Protein Exchange. Medicine, Moscow, 168 p.